CLEC7A Antibody | 6833

CLEC7A Antibody | 6833 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (88%)

Immunogen: CLEC7A antibody was raised against a 17 amino acid synthetic peptide near the center of human CLEC7A.
The immunogen is located within amino acids 70 - 120 of CLEC7A.

Research Area: Innate Immunity

Tested Application: E, WB, IHC-P, IF

Application: CLEC7A antibody can be used for detection of CLEC7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Multiple isoforms of CLEC7A are known to exist.

Positive Control 1: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 27 kDa
Observed: 27 kDa

Validation: N/A

Isoform: N/A

Purification: CLEC7A Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CLEC7A Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CLEC7A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: CLEC7A Antibody: BGR, CANDF4, DECTIN1, CLECSF12, BGR, UNQ539/PRO1082, C-type lectin domain family 7 member A, Beta-glucan receptor, DC-associated C-type lectin 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CLEC7A Antibody: CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM) . CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1, 3-linked and beta-1, 6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.