Description
Clusterin Antibody | 45-407 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: Expected Species Reactivity based on sequence homology: Dog
Immunogen: The immunogen for this antibody is: C-EKALQEYRKKHREE
Research Area: Cancer
Tested Application: E, WB, IF
Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 35-37kDa band observed in Human Colon and Prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1) . The observed molecular weight corresponds to earlier findings in literature (PMID: 9713325 and PMID: 11560857) . Recommended concentration: 0.03-0.1ug/ml. Primary incubation was 1 hour.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10ug/ml
Specificiy: This antibody is expected to recognise both reported isoforms (NP_001822.2 and NP_976084.1)
Positive Control 1: Cat. No. 1320 - Human Colon Tissue Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: CLU, APOJ, clusterin, complement lysis inhibitor, SP-40, 40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J, HGNC:2095, CLI, MGC24903, SGP-2, SGP2, SP-40, TRPM-2, TRPM2, apolipoprotein J, clusterin, complement-associated protein SP-40, sulfated glycoprotein 2, AAG4, KUB1, aging-associated protein 4, clusterin (complement lysis inhibitor, SP-40, 40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J)
User Note: N/A
BACKGROUND: N/A