CNTF/Ciliary neurotrophic factor | E21-098

CNTF/Ciliary neurotrophic factor | E21-098 | Gentaur UK, US & Europe Distribution

Short Description: Recombinant Human Ciliary Neurotrophic Factor is produced by our E.coli expression system and the target gene encoding Ala2-Met200 is expressed.

Shipping: Ambient

Formulation: Lyophilized from a 0.2 m filtered solution of 20mM PB, 150mM NaCl, 1mM Cysteine, pH 7.4.

Storage: Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Reconstitution: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 g /ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/g (1 IEU/g) as determined by LAL test.

Background: Ciliary Neurotrophic Factor (CNTF) is a potent survival factor for neurons and oligodendrocytes. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL6, IL11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal transducing subunit in their receptor complexes. CNTF, like FGF acidic, FGF basic, and PD-ECGF (platelet-derived endothelial cell growth factor) , does not possess a signal sequence that would allow secretion of the factor by classical secretion pathways. The mechanism underlying the release of CNTF is unknown.

Species: Human

Expression System: E.coli