CNTF, rat recombinant | 4019

CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. CNTF is a survival factor for various neuronal cell types and seems to prevent the degeneration of motor axons after axotomy. CNTF Recombinant Rat produced in E.Coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 22834 Dalton. The CNTF is purified by proprietary chromatographic techniques.

4019 | CNTF rat recombinant DataSheet

Biomolecule/Target: CNTF

Synonyms: HCNTF, CNTF, Ciliary Neurotrophic Factor.

Alternates names: Bone morphogenetic protein 5, BMP-5, BMP5, MGC34244

Taglines: A potent neural factor promoting survivability and differentiation of neuronal cell types.

NCBI Gene ID #: 653

NCBI Gene Symbol: BMP5

Gene Source: Human

Accession #: P22003

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: n/a

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent induction of alkaline phosphatase production with ATDC5 chondrogenic cells is typically 0.3-3 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 31.0 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Lyophilized from 0.025 % NaHCO

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 ug BSA per 1 ug of protein). This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20° for future use.

Amino acid sequence: N/A