Coelenterazine, native | 2536

Cell-permeable. Coelenterazine is the luminescent substrate for Renilla luciferase and apoaequorin. When coelenterazine combines with luciferase, oxygen, and calcium ions, it produces oxy-coelenterazine and light. This allows coelenterazine to be used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.

Coelenterazine is the luminescent substrate for Renilla luciferase and apoaequorin. When coelenterazine combines with luciferase, oxygen, and calcium ions, it produces oxy-coelenterazine and light. This allows coelenterazine to be used for bioluminescent calcium detection and the monitoring of reporter genes in BRET (Bioluminescence Resonance Energy Transfer), ELISA and HTS methods as well as chemiluminescent detection of superoxide anions and peroxynitrite in cells or tissues.

2536 | Coelenterazine, native DataSheet

Alternate Name/Synonyms: 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN

Appearance: Brownish yellow solid

Formulation: N/A

CAS Number: 55779-48-1

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₆H₂₁N₃O₃

Molecular Weight: 423.46

Cell-Permeable?: Yes

Purity: ≥95% by TLC

Solubilities: EtOH or MeOH

Handling: Protect from air and moisture

Country of Origin: USA

Tag Line: A luminescent substrate

MDL Number: MFCD00467176

PubChem CID: 2830

SMILES: C1=CC=C(C=C1)CC2=C3N=C(C(=O)N3C=C(N2)C4=CC=C(C=C4)O)CC5=CC=C(C=C5)O

InChi: InChI=1S/C26H21N3O3/c30-20-10-6-18(7-11-20)15-23-26(32)29-16-24(19-8-12-21(31)13-9-19)27-22(25(29)28-23)14-17-4-2-1-3-5-17/h1-13,16,27,30-31H,14-15H2

InChi Key: YHIPILPTUVMWQT-UHFFFAOYSA-N