Cofilin (phospho-S3) polyclonal Antibody | BS4716

Cofilin (phospho-S3) polyclonal Antibody | BS4716 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Cofilin (S3) polyclonal Antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.

Molecular Weight: ~ 19 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Cofilin-1; 18 kDa phosphoprotein; p18; Cofilin; non-muscle isoform; CFL1; CFL

Immunogen: Synthetic phosphopeptide derived from human Cofilin 1 around the phosphorylation site of Serine 3.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Contribution of ECM and Cytoskeletal Factors to EMT,B cell receptor signaling,