Collagen-Fluorescein (FITC) Conjugate | M1304

Collagen is a major component of the extracellular matrix in vertebrates and it constitutes approximately 25% of total protein. It plays an important in cell adhesion and migration. Specific collagen receptors, fibronectin and a number of other proteins involved in cell–cell and cell–surface adhesion have been demonstrated to bind collagen and gelatin (denatured collagen). ’s Collagen-FITC is heavily modified collagen with a fluorophore (fluorescein Isothiocyanate, FITC). The conjugate is a highly quenched substrate for gelatinases/collagenases. It can be digested by gelatinases, collagenases and other matrix degrading metalloproteases, releasing brightly fluorescent peptides (Ex/Em = 490/520 nm). The increase in fluorescence upon digestion is proportional to the proteolytic activity of the enzyme under investigation. ’s Collagen-FITC can also be used to detect collagen binding proteins and in live cell staining.

M1304 | Collagen-Fluorescein (FITC) Conjugate DataSheet

Sort Name: Collagen-Fluorescein (FITC) Conjugate

Label Name: Collagen-Fluorescein (FITC) Conjugate

Taglines: An ideal substrate for detection of collagen binding proteins and live cell staining

Product Highlights: FORM: Lyophilized FORMULATION: 5 mg/ml protein in TBS STORAGE CONDITIONS: Stable for one year at -20°C APPLICATIONS/BENEFITS: • Substrate for detection of Gelatinases/Collagenase and other gelatin degrading enzymes •To study collagen binding proteins • Live cell staining

Packaging: EA