Additional Information
Size: |
100 µl |
Target: |
Collagen I |
Conjugate: |
HRP |
Research Area: |
Cell Signaling | Extracellular Matrix (ECM) | Collagen | Stem Cells | Mesenchymal Stem Cells | Osteogenesis |
Alternative Name: |
Alpha 1 type I collagen Antibody, Alpha 2 type I collagen Antibody, CO1A1_HUMAN Antibody, collagen alpha 1 chain type I Antibody, EDSC Antibody, Type I procollagen antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthesized peptide derived from N-terminal of human Collagen I. |
Immunogen Species: |
Human |
Applications: |
IHC, ICC/IF, ELISA |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
IHC (1:100), ICC/IF (1:500), ELISA (1:5000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects ~139kDa, endogenous levels of total Collagen I protein. Collage type I is a triple helix consisting of one alpha-2 and 2 alpha-1 chains, so dimeric (~270kDa) and trimeric (~400kDa) forms may be expected. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:100 dilution of SPC-1273 was sufficient for detection of Collagen I in NIH/3T3 cells using Goat Anti-Rabbit IgG as the secondary antibody. |
Cellular Localization: |
Extracellular matrix |
Tissue Specificity: |
Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite. |
Accession Number: |
NP_000080.2 |
Gene ID: |
1278 |
Swiss-Prot: |
P08123 |
Field of Use: |
For in vitro research use only. |
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