Additional Information
Size: |
100 µl |
Target: |
Collagen II |
Conjugate: |
PE/ATTO 594 |
Research Area: |
Cell Signaling | Extracellular Matrix (ECM) | Collagen | Stem Cells | Mesenchymal Stem Cells | Osteogenesis | Cell Signaling | Extracellular Matrix (ECM) | Bone |
Alternative Name: |
Alpha 1 type II collagen Antibody, AOM Antibody, Cartilage collagen Antibody, Chondrocalcin Antibody, COL11A3 Antibody, Collagen II alpha 1 polypeptide Antibody, SEDC Antibody |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthesized peptide derived from human Collagen II. |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ICC/IF, ELISA |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human, Mouse, Rat |
Antibody Dilution: |
WB (1:1000), IHC (1:100), ICC/IF (1:500), ELISA (1:20000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of total Collagen II protein. |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:1000 dilution of SPC-1274 was sufficient for detection of Collagen II in 10 µg of COLO205 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody. |
Cellular Localization: |
Extracellular matrix |
Tissue Specificity: |
Isoform 2 is highly expressed in juvenile chondrocyte and low in fetal chondrocyte. |
Accession Number: |
NP_149162.2 |
Gene ID: |
1280 |
Swiss-Prot: |
P02458 |
Field of Use: |
For in vitro research use only. |