Colon Tissue Lysate (Tumor) | 1716-03

Colon Tissue Lysate (Tumor) | 1716-03 | Gentaur UK, US & Europe Distribution

Tested Application: WB

Application: N/A

Storage Condition: Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.

User Note:

Background:

Shipping: N/A

Type: Tissue

Physical State: Liquid

Buffer: Colon Tissue Lysate (Tumor) is supplied in SDS sample buffer containing 5% β-mercaptoethanol.

Concentration: 2 mg/mL

Background: Colon tumor tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.

Disclaimer: This product is intended for laboratory research purposes only and should be used by qualified personnel only. ProSci lysates are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci lysates.

Short Description: total protein colon tumor - human

Source: Human Colon Tissue

Tissus By Diagnosis: Diseased

Tissus By Developemental Stage : N/A

Tissus By Species: Human

Tissus/ Cell: Colon

Diagnostic: Tumor

Cancer Type: Invasive adenocarcinoma

Cancer Type Ezpanded: moderately differentiated

Age: 58

Sex: M

Race: Caucasian

Species: Human