Complement C4c, Human Plasma | P1272

Complement C4 (C4) plays a central role in both the classical and lectin pathways of complement activation, with levels in human plasma ~0.4 mg/ml. C4 circulates in human plasma as a heterotrimer of 205,000 Da in plasma. The three disulfide-linked chains have molecular weights of 93 (alpha), 75 (beta), and 32 (gamma) kDa. These are readily processed to create C4a, C4b or C4c depending on the processed state. Complement C4c is a degradation product of C4b which is cleaved by C4b/C3b inactivator to yield C4d and C4c. Complement C4c interacts with C1 and C2 to form C3 convertase of the classic activation pathway. Systemic lupus erythematosus (SLE) is often associated with congenital C4 deficiency. Reduced levels of components of the classic pathway (C1, C2, C4, C3) are common after such activation, e.g. in SLE, acute serum sickness and conditions associated with circulating immune complexes

P1272 | Complement C4c Human Plasma DataSheet

Biomolecule/Target: C4c

Synonyms: Compelement C4c

Alternates names: bone morphogenetic protein 11, BMP11, BMP 11, Growth/Differentiation Factor-11, GDF-11, GDF11, GDF 11

Taglines: A key component of the complement system

NCBI Gene ID #: 10220

NCBI Gene Symbol: BMP11/GDF11

Gene Source: Human

Accession #: O95390

Recombinant: No

Source: Human Plasma

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by C2C12 cells. The ED is in the range of 0.4-4 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 32.6 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In PBS, pH 7.4

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 g BSA per 1 g of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A