Complement System Classical Pathway is an immunoassay for the qualitative and/or semi-quantitative determination of functional classical complement pathway and determination of complement deficiencies in human serum.

 Complement Classical Pathway assay combines the specific activation of the pathway with the use of labeled antibodies specific for a neoepitope of the terminal complement complex, C5b-9, produced as the result of complement activation. Objective quantification of the activity of the classical pathway is valuable in a number of different clinical situations. The amount of complement activation can be expressed either in a qualitative way relative the value of a positive control or in a semi-quantitative way using a calibrator curve derived from a separate lyophilized activity control.

What's in the box?

• One frame with break-apart wells (12x8)
• 2 x 35 mL Diluent CP
• 13 mL Conjugate
• 13 mL Substrate solution
• 30 mL Wash solution 30x concentrated
• 0,2 mL Negative control (NC) containing human serum
• Lyophilized positive control (PC) containing human serum
• Lyophilized activity control (AC) for semi-quantitative application, containing human serum

Please note: All reagents in the kit are ready to use except wash solution and controls. The reagents should be stored at 2-8° C. The positive control and the activity control should be stored at -20° C upon arrival. The reconstituted positive control and activity control should be stored at –70° C and may be thawed once.