Control Normal llama IgG3 (FITC) | XR-8007

Control Normal llama IgG3 (FITC) | XR-8007 | Gentaur UK, US & Europe Distribution

Host : Llama

Reactivity : N/A

Tested Application : E, WB, IF, Flow

Application: Llama IgG3 FITC conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

Isotype: IgG3

Physical State: Lyophilized

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Concentration: 1 mg/mL

Storage Condition: Store vial at 4˚C prior to restoration. For extended storage aliquot contents and freeze at -20˚C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Restore with 100 uL deionized water (or equivalent)

Background: Comparative studies of old world camelids (Camelus bactrianus and Camelus dromedarius) and new world camelids (Lama pacos, Lama glama and Lama vicugna) have shown that heavy-chain-only immunoglobulins represent between 35% - 70% of total IgG in the sera of all species. Such antibodies are homodimers of heavy chains that lack the CH1 domain of conventional antibodies and therefore do not interact with light chains, exhibiting a lower molecular weight ~100 kDa. In llama and other species of camelids, these heavy-chain-only immunoglobulins belong to the IgG2 and IgG3 subclasses of gamma chain antibodies.