Core Histones (Human) | 7677

A nucleosome is the basic repeating unit of chromatin in which 146 base pairs of DNA wrap twice around a histone octamer consisting of two copies of each of the core histones, H2A, H2B, H3, and H4. The combination of two H2A/H2B dimers and one H3/H4 tetramer create the nucleosome core.2 Histone H3 undergoes many modifications which include acetylation, methylation, and phosphorylation that are important for regulation of gene transcription.

7677 | Core Histones Human DataSheet

Biomolecule/Target: Core Histones

Synonyms: Core Histones (Human)

Alternates names: bone morphogenetic protein 11, BMP11, BMP 11, Growth/Differentiation Factor-11, GDF-11, GDF11, GDF 11

Taglines: Mixture of human core histones involved in gene transcription

NCBI Gene ID #: 10220

NCBI Gene Symbol: BMP11/GDF11

Gene Source: Human

Accession #: O95390

Recombinant: No

Source: Highly purified mixture of human core histones (H2A, H2B, H3, and H4) isolated via hydroxyapatite chromatography from HeLa S3 (human cervical adenocarcinoma) nuclear pellet.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by C2C12 cells. The ED is in the range of 0.4-4 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 32.6 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 50 mM sodium phosphate, pH 6.9, containing 10 mM sodium chloride and 1 mM -mercaptoethanol.

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 g BSA per 1 g of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A