Cortactin (phospho-Y421) polyclonal Antibody | BS4778

Cortactin (phospho-Y421) polyclonal Antibody | BS4778 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB IHC

Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200

Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486) . In vitro, cortactin binds to and cross links F actin into meshworks.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: p-Cortactin (Y421) polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr421.

Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1

Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.

Conjugate: Unconjugated

Modification: Phosphorylation

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Regulation of Actin Dynamics,Contribution of ECM and Cytoskeletal Factors to EMT,Adherens Junction signaling,