BW
Cortactin (phospho-Y466) polyclonal Antibody | BS4779
- SKU:
- BW-BS4779
- Availability:
- Usually ships in 5 working days
Description
Cortactin (phospho-Y466) polyclonal Antibody | BS4779 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human,Mouse,Rat
Application: WB IHC
Application Range: WB: 1:500~1:1000 IHC: 1:50~1:200
Background: Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486) . In vitro, cortactin binds to and cross links F actin into meshworks.
Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity: p-Cortactin (Y466) polyclonal Antibody detects endogenous levels of Cortactin protein only when phosphorylated at Tyr466.
Molecular Weight: ~ 85 kDa
Note: For research use only, not for use in diagnostic procedure.
Alternative Names: Src substrate cortactin; Amplaxin; Oncogene EMS1; CTTN; EMS1
Immunogen: Synthetic peptide, corresponding to amino acids 360-418 of Human TFDP1.
Conjugate: Unconjugated
Modification: Phosphorylation
Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Pathway: