Coxiella burnetii RT-PCR (CE) | B85-50FRT

Coxiella burnetii RT-PCR | B85-50FRT is available for delivery

Description:

General information: Real Time Amplification kit

Target Disease Type: Dangerous Infections

Specific Application: Coxiella burnetii

Storage and Shipping : stock

Coxiella burnetii RT-PCR (CE) B85-50FRT DataSheet

INTRODUCTION

Q fever is a zoonosis caused by Coxiella burnetii, an obligate gram-negative intracellular bacterium. Most commonly reported in southern France and Australia, Q fever occurs worldwide. C burnetii infects various hosts, including humans, ruminants (cattle, sheep, goats), and pets. In rare cases, C burnetii infection in reptiles, birds, and ticks has been reported. C burnetii is excreted in urine, milk, feces, and birth products. These products, especially the latter, contain large numbers of bacteria that become aerosolized after drying. Coxiella burnetiiis a highly infectious agent that is rather resistant to heat and drying. It can become airborne and inhaled by humans. A single C. burnetii organism may cause disease in a susceptible person. This agent could be developed for use in biological warfare and is considered a potential terrorist threat.

Molecular detection by PCR is unaffected by changes in the infection cycle, and enables rapid, sensitive and specific detection of C. burnetii in a sample.

INTENDED USE

The Coxiella burnetii Real-TM is a “Real-Time Amplification” test for the qualitative detection of C.burnetii in the human and animal biological materials (whole blood, urine, feces, tissue, milk, etc) and in the environment (soil, water). C.burnetii DNA is extracted from samples, amplified using real time amplification with fluorescent reporter dye probes specific for C.burnetii and Internal Control ( IC).

PRINCIPLE OF ASSAY

In Coxiella burnetii Real-TM kit there are 2 independent reactions running in parallel in each tube: the first detects specific fragment of C.burnetii and the second detects internal control (IC) DNA which allows excluding unreliable results. Positive result of reference reaction 2 (IC) together with negative results of reactions 1 (C.burnetii) confirm the absence of C.burnetii in a sample. A negative result of both reactions is a sign of PCR inhibition, and this provides a way to avoid false-negative results.