CPEB1 Antibody (C-term) [APR11651G]

CPEB1 Antibody (C-term) [APR11651G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CPEB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 447-475 amino acids from the C-terminal region of human CPEB1.

Cellular Localisation: Cytoplasm. Nucleus Cytoplasm, P-body. Cytoplasmic granule. Cell junction, synapse Membrane. Cell junction, synapse, postsynaptic density Cell projection, dendrite. Note=Continuously shuttling between nucleus and cytoplasm (PubMed:18923137) . Also found in stress granules. Recruited to stress granules (SGs) upon arsenite treatment In dendrites (By similarity) . Localizes in synaptosomes at dendritic synapses of neurons (By similarity) . Strongly enriched in postsynaptic density (PSD) fractions (By similarity) . Transported into dendrites in a microtubule-dependent fashion and colocalizes in mRNA-containing particles with TACC3, dynein and kinesin (By similarity) . Membrane- associated (By similarity) . Colocalizes at excitatory synapses with members of the polyadenylation and translation complex factors (CPSF, APLP1, TACC3, AURKA, SYP, etc.) including CPE-containing RNAs (By similarity) . {ECO:0000250, ECO:0000269|PubMed:18923137}

Molecular Weight: 62595

Clone: Polyclonal

Gene Name: CPEB1

Gene ID: 64506

Function: Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the mRNA 3'- UTR. RNA binding results in a clear conformational change analogous to the Venus fly trap mechanism (PubMed:24990967) . In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity) . Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses (By similarity) . Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation (By similarity) . Induces the assembly of stress granules in the absence of stress. Required for cell cycle progression, specifically for prophase entry (PubMed:26398195) .

Summary: Tissue Location: Isoform 1 is expressed in immature oocytes, ovary, brain and heart. Isoform 2 is expressed in brain and heart. Isoform 3 and isoform 4 are expressed in brain. Expressed in breast tumors and several tumor cell lines.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Cytoplasmic polyadenylation element-binding protein 1, CPE-BP1, CPE-binding protein 1, h-CEBP, hCPEB-1, CPEB1, CPEB