CRBN Antibody | 8115

CRBN Antibody | 8115 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (94%)

Immunogen: CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN.
The immunogen is located within the last 50 amino acids of CRBN.

Research Area: Neuroscience, Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: CRBN antibody can be used for detection of CRBN by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: CRBN antibody is human, mouse and rat reactive. At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 49 kDa
Observed: 54 kDa

Validation: N/A

Isoform: N/A

Purification: CRBN antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CRBN antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CRBN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Cereblon, AD-006, DKFZp781K0715, MGC27358, MRT2A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CRBN, a member of the Lon protease protein family, plays a role in brain development (1) . It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8 (2) . CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain (3, 4) .