Creatine Kinase MT, Human Recombinant | P1578

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Recombinant human CKMT1A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

P1578 | Creatine Kinase MT, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Creatine kinase U-type, mitochondrial, Acidic-type mitochondrial creatine kinase, Mia-CK, Ubiquitous mitochondrial creatine kinase, U-MtCK, CKMT1A, CKMT

Alternates names: Creatine kinase U-type, mitochondrial, Acidic-type mitochondrial creatine kinase, Mia-CK, Ubiquitous mitochondrial creatine kinase, U-MtCK, CKMT1A, CKMT

Taglines: Plays a central role in energy transduction

NCBI Gene ID #: 1159

NCBI Gene Symbol: CKMT1A, CKMT1B

Gene Source: Human

Accession #: P12532

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): > 50 unit/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit is defined as the amount of enzyme that converts 1.0 umole of phosphate from phosphocreatine to ADP per minute at pH 7.5 at 37C.

Molecular Weight: 45 kDa (403aa) (N-terminus His-Tag)

Concentration:

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol

Reconstitution Instructions:

Amino acid sequence: aa 40-417

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans