CREB Binding Protein bromodomain (1081-1197 aa) (GST-tagged), Human recombinant | 7659

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The cAMP response element-binding protein (CREB) binding protein (CREBBP) bromodomain has been shown to modulate the stability and function of the tumor suppressor protein p53. CREBBP bromodomain recognizes the acetylated lysine residue 382 on p53. This product contains the bromodomain region of CREBBP.

7659 | CREB Binding Protein bromodomain 1081-1197 aa GST-tagged Human recombinant DataSheet

Biomolecule/Target: CREBBP

Synonyms: cAMP-responsive element-binding protein 1 CREB-1; CBP; CREBBP

Alternates names: BMP-2, BMP2A, bone morphogenetic protein2

Taglines: A bromodomain modulating the stability and function of tumor suppressor protein p53

NCBI Gene ID #: 650

NCBI Gene Symbol: BMP 2

Gene Source: Human

Accession #: P12643

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED50 as determined by the dose-dependent cytolysis of ADTC cells is 2.8 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 26.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM -mercaptoethanol and 5% glycerol.

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 ug BSA per 1 ug of protein). This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A