CRISPR-Cas9 Mouse monoclonal Antibody | MB9030

CRISPR-Cas9 Mouse monoclonal Antibody | MB9030 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Recombinant Cas9

Application: WB ICC

Application Range: WB: 1:1000-5000 ICC: 1:50-200

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) . CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable) . In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects recombinant Cas9 protein.

Molecular Weight: ~ 158 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9

Immunogen: This Antibody is produced by immunizing mice with a recombinant protein corresponding to a sequence of CRISPR/Cas9.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Protein A affinity purified

Pathway: