CRISPR-Cas9 SP polyclonal Antibody | BS90331

CRISPR-Cas9 SP polyclonal Antibody | BS90331 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Streptococcus pyogenes

Application: WB ICC/IF IHC FC

Application Range: WB:1:1,000-1:5,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100

Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids) . CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable) . In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Specificity: CRISPR-Cas9 SP polyclonal Antibody detects endogenous levels of CRISPR-Cas9 SP protein.

Molecular Weight: 158 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: cas9, Cas9, CRISPR-associated endonuclease Cas9/Csn1, CRISPR-Cas9/Csn1, csn1, SpyCas9,

Immunogen: recombinant protein

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: