CRUM2 Antibody (C-term) [APR32192G]

CRUM2 Antibody (C-term) [APR32192G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This CRUM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1258-1285 amino acids of human CRUM2.

Cellular Localisation: [Isoform 1]: Apical cell membrane {ECO:0000250|UniProtKB:Q80YA8}; Single-pass type I membrane protein. Cytoplasm {ECO:0000250|UniProtKB:Q80YA8}. Cell junction {ECO:0000250|UniProtKB:Q80YA8}. Note=O-glucosylation is required for localization at the apical plasma membrane (By similarity) . Distributed in a complex anisotropic pattern on apical cell edges: the level of CRB2 on a cell edge is inversely correlated with the level of MYH10/myosin-IIB (By similarity) . {ECO:0000250|UniProtKB:Q80YA8}

Molecular Weight: 134265

Clone: Polyclonal

Gene Name: CRB2 (HGNC:18688)

Gene ID: 286204

Function: Apical polarity protein that plays a central role during the epithelial-to-mesenchymal transition (EMT) at gastrulation, when newly specified mesodermal cells move inside the embryo (By similarity) . Acts by promoting cell ingression, the process by which cells leave the epithelial epiblast and move inside the embryo to form a new tissue layer (By similarity) . The anisotropic distribution of CRB2 and MYH10/myosin-IIB at cell edges define which cells will ingress: cells with high apical CRB2 are probably extruded from the epiblast by neighboring cells with high levels of apical MYH10/myosin-IIB (By similarity) . Plays a role in the maintenance of retinal neuroepithelium organization, structural integrity, adhesion, photoreceptor polarity and retinal photoreceptor layer thickness (By similarity) . May play a role in determining the length of cone photoreceptor outer segments and proliferation of late-born progenitor cells (By similarity) . Also required for maintenance of the apical polarity complex during development of the cortex (By similarity) . Inhibits gamma-secretase- dependent cleavage of APP and secretion of amyloid-beta peptide 40 and amyloid-beta peptide 42, and thereby inhibits gamma-secretase-dependent Notch transcription (PubMed:20299451) .

Summary: Tissue Location: Expressed in glomeruli, podocytes of the glomerular capillary loops, and parietal glomerular epithelial cells in the kidney (at protein level) (PubMed:29473663, PubMed:27942854) . Expressed in retina, fetal eye and brain (PubMed:15851977) . Also expressed in kidney, RPE/choroid, and at low levels in lung, placenta, and heart (PubMed:15851977) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: Protein crumbs homolog 2, Crumbs-like protein 2, CRB2