CSK, Active | 7722

CSK is a cytoplasmic tyrosine kinase that has been shown to downregulate the tyrosine kinase activity of the c-src oncoprotein through tyrosine phosphorylation of the c-src carboxy terminus (1). Cell transformation by src oncoprotein is caused by several oncogenic mechanisms, which interfere with the carboxy terminal phosphorylation. The CSK could therefore potentially function as an anti-oncogene. CSK is ubiquitously expressed in human tissues as 2 mRNA species of 2.6 and 3.4 kb, although in some tissues and cell lines, only the larger mRNA is detected (2). A yeast 2-hybrid system has been used to identify proteins associated with CSK. The Src homology-3 (SH3) domain of CSK associates with a proline-rich region of PEP, a protein-tyrosine phosphatase expressed in hemopoietic cells (2). This association is highly specific and it is speculated that PEP may be an effector and/or regulator of CSK in T cells and other hemopoietic cells. An in situ hybridization has been used to map the CSK gene to chromosome 15q23-q25 (3).

7722 | CSK Active DataSheet

Biomolecule/Target: N/A

Synonyms: CSK, C-src tyrosine kinase

Alternates names: CSK, C-src tyrosine kinase, Protein-tyrosine kinase CYL

Taglines: Phosphorylates human Src

NCBI Gene ID #: 1270

NCBI Gene Symbol: CNTF

Gene Source: N/A

Accession #: P26441

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 425 nmol phosphate incorporated into Poly(Glu-Tyr) per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 78.0 kDa

Concentration: 5µg/50 µl

Appearance: Lyophilized powder

Physical form description: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, pH 7.4, 5% trehalose and 5% mannitol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A