CTCF Antibody | 14-510

CTCF Antibody | 14-510 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_006556.1) .

Research Area: Other

Tested Application: WB, IHC, IP, ChIP

Application: WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IP: 1:50 - 1:100
ChIP: 1:20 - 1:50

Specificiy: N/A

Positive Control 1: A-549

Positive Control 2: HeLa

Positive Control 3: Jurkat

Positive Control 4: U-251MG

Positive Control 5: 293T

Positive Control 6: Mouse brain

Molecular Weight: Observed: 131kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: CTCF, MRD21

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT) -containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC) -containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.