CTGFL/WISP-2, Human Recombinant | 7129

CTGFL/WISP-2 is a 28.6 kDa protein that belongs to the CCN family of cysteine rich regulatory proteins. Members of this family stimulate mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types. The protein is expressed in primary osteoblasts, fibroblasts, ovary, testes, and heart. In addition to promoting adhesion of osteoblasts, CTGFL/WISP-2 inhibits osteocalcin production, as well as binding of fibrinogen to integrin receptors. Recombinant human CTGFL/WISP-2 is a 24.3 kDa protein of 228 amino acid residues.

7129 | CTGFL/WISP-2 Human Recombinant DataSheet

Biomolecule/Target: CTGFL/WISP-2

Synonyms: Connective Tissue Growth Factor-Like protein, CCN5, CT-58

Alternates names: Cartilage-derived morphogenetic protein-1, CDMP-1, LAP4, SYNS2, GDF-5, Radotermin, CDMP1, GDF5, Growth differentiation factor 5, BMP-14

Taglines: Stimulates mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest, and migration of multiple cell types

NCBI Gene ID #: 8200

NCBI Gene Symbol: GDF5

Gene Source: Human

Accession #: X80915

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to inhibit IGF-II induced proliferation of MCF-7. The expected ED50 is between 10-20 ng/ml in the presence of 15 ng/ml of human IGF-II.

Biological activity: The ED as determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells is 1-2 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 13.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers.

Amino acid sequence: N/A