cTnI Antibody [3A10A12] | 32-141

cTnI Antibody [3A10A12] | 32-141 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3) .

Research Area: Other

Tested Application: E, WB, IHC

Application: Western Blot:Dilution 1:500 - 1:2, 000
IHC (P) :Dilution 1:500 - 2, 000
IHC (F) :Dilution 1:500 - 2, 000
ELISA:Propose dilution 1:10, 000.
Determining optimal working dilutions by titration test.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: 3A10A12

Isotype: IgG1

Conjugate: Unconjugated

Physical State: N/A

Buffer: Ascitic fluid containing 0.03% sodium azide.

Concentration: N/A

Storage Condition: cTnI monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: TNNC1, Cardiac troponin ICMH7, RCM1, cTnI, CMD2A, TNNC1, CMD1FF

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin) , thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.