CTSB, mouse recombinant | P1095

CTSB, also known as cathepsin B preproprotein, is a papain-family cysteine protease that is normally located in lysosomes. It has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Recombinant mouse CTSB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1095 | CTSB mouse recombinant DataSheet

Biomolecule/Target: CTSB

Synonyms: Cathepsin B, Ctsb, CB

Alternates names: Cathepsin B, Ctsb, CB

Taglines: An anti-protease implicated in myoclonus epilepsy.

NCBI Gene ID #: 1270

NCBI Gene Symbol: CNTF

Gene Source: Mouse

Accession #: P26441

Recombinant: Yes

Source: Insect cell (Baculovirus)

Purity by SDS-PAGEs: 99%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): Measured by the hydrolysis of 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

Biological activity: Specific activity is >2,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

Results: >2,000 pmol/min/ug

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1pmole of Z-Arg-Arg-AMC to Z-Arg-Arg and AMC per minute at pH 6.0 at 37C.

Molecular Weight: 36.4 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol

Reconstitution Instructions: N/A

Amino acid sequence: HDKPSFHPLS DDLINYINKQ NTTWQAGRNF YNVDISYLKK LCGTVLGGPK LPGRVAFGED IDLPETFDAR EQWSNCPTIG QIRDQGSCGS CWAFGAVEAI SDRTCIHTNG RVNVEVSAED LLTCCGIQCG DGCNGGYPSG AWSFWTKKGL VSGGVYNSHV GCLPYTIPPC EHHVNGSRPP CTGEGDTPRC NKSCEAGYSP SYKEDKHFGY TSYSVSNSVK EIMAEIYKNG PVEGAFTVFS DFLTYKSGVY KHEAGDMMGG HAIRILGWGV ENGVPYWLAA NSWNLDWGDN GFFKILRGEN HCGIESEIVA GIPRTDQYWG RFLEHHHHHH