Cyan Fluorescent Protein | 4996

The recombinant CFP (Cyan Fluorescent Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal CFP fluorescence. Endotoxin has been removed, so the protein is suitable for in vivo injection or cell culture applications. The protein is a 31.3 kDa monomer with 284 amino acids and an N-terminal His-tag, Ex./Em. = 458/480 nm, extinction coefficient 26000M-1CM-1.

4996 | Cyan Fluorescent Protein DataSheet

Biomolecule/Target: N/A

Synonyms: Recombinant CFP Protein, Cyan Fluoroscent Protein

Alternates names: bone morphogenetic protein 10, BMP10, BMP 10

Taglines: Fluorescent protein ideal for subcellular labeling/visualization

NCBI Gene ID #: 27302

NCBI Gene Symbol: BMP10

Gene Source: N/A

Accession #: O95393

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 97%

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): N/A

Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by C2C12 cells. The ED is in the range of 0.3-3 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 32 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Freeze Dried

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A