Cyclic-di-GMP Assay Kit - K1483

K1483 | Cyclic-di-GMP Assay Kit DataSheet

’s Cyclic-di-Guanosine Monophosphate (cyclic-di-GMP) Assay Kit is a simple, rapid, highly selective assay to measure cyclic-di-GMP levels in cells. This assay can be used to detect cyclic-di-GMP in any biochemical or enzymatic reaction that produces cyclic-di-GMP or in cell-based applications to monitor intracellular cyclic-di-GMP concentrations. In this assay, the evolution of the fluorescent signal is dependent on the initial binding of cyclic-di-GMP to the cyclic-di-GMP riboswitch in the sensor. This results in the stabilization of the SpinachTM aptamer, which in turn binds to the DFHBI-1T Fluorophore to produce a stable fluorescence, that can be measured at Ex/Em = 482/505 nm. The assay is easy to use and is homogenous. The end user needs to simply incubate the samples with the provided assay reagents for 30 min followed by reading the samples on a fluorescence plate reader at Ex/Em = 482/505 nm.

Alternate Name : Cyclic-di-Guanosine Monophosphate Kit,Cyclic-di-GMP Assay,Cyclic-di-GMP Assay Kit,Cyclic-di-GMP Kit,Cyclic-diGMP Assay Kit,Cyclic-di-Guanosine Monophosphate Assay,cGMP Assay Kit,Cyclic-di-Guanosine Monophosphate Assay Kit

Tag Line : Aptamer based assay to measure cyclic-di-GMP levels in cells or in any biochemical or enzymatic reaction

Summary : ’s Cyclic-di-Guanosine Monophosphate (cyclic-di-GMP) Assay Kit is a simple, rapid, highly selective assay to measure cyclic-di-GMP levels in cells. This assay can be used to detect cyclic-di-GMP in any biochemical or enzymatic reaction that produces cyclic-di-GMP or in cell-based applications to monitor intracellular cyclic-di-GMP concentrations.

Detection Method : Fluorescence at Ex/Em = 482/505 nm

Sample Type : Biochemical samples,Bacterial cells

Species Reactivity :

Applications : To detect cyclic-di-GMP in any biochemical or enzymatic reaction that produces cyclic-di-GMP.,Cell-based applications to monitor intracellular cyclic-di-GMP concentrations in bacteria.

Features and Benefits : A simple, rapid, highly selective assay to measure cyclic-di-GMP levels in cells.,The end user needs to simply incubate the samples with the provided assay reagents for 30 min followed by reading the samples on a fluorescence plate reader at Ex/Em = 482/505 nm.,The assay is easy to use and is homogenous.