Cyclin-dependent kinase inhibitor 2A, Human Recombinant | P1008

Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein. This activity has the effect of suppressing tumor formation and growth, and of inducing replicative senescence in various normal cells, including stem cells. The expression of Cyclin-Dependent Kinase Inhibitor 2A steadily increases with age, and tends to accumulate in stem cell compartments. The deletion, rearrangement, or mutation of the Cyclin-Dependent Kinase Inhibitor 2A gene is frequently found in melanomas, as well as in certain other types of cancer.

P1008 | Cyclin-dependent kinase inhibitor 2A Human Recombinant DataSheet

Biomolecule/Target: Cyclin-dependent kinase inhibitor 2A

Synonyms: p16-INK4a, Cyclin-Dependent Kinase Inhibitor 2A, Cyclin-Dependent Kinase 4 Inhibitor A, CDK4I, p16INK4A, p16-INK4, Multiple Tumor Suppressor 1, MTS-1

Alternates names: bone morphogenetic protein 10, BMP10, BMP 10

Taglines: A negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6

NCBI Gene ID #: 27302

NCBI Gene Symbol: BMP10

Gene Source: Human

Accession #: O95393

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 95%

Assay #2: HPLC

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): N/A

Biological activity: The protein is biologically active as determined by its ability to induce alkaline phosphatase production by C2C12 cells. The ED is in the range of 0.3-3 µg/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 32 kDa

Concentration: N/A

Appearance: Dry powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute to a concentration of 0.1-1.0 mg/ml in HO containing BSA (50 mg BSA per 1 mg of protein). This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A