Additional Information
Size: |
100 µl |
Target: |
Cyclin E1 (pThr395) |
Conjugate: |
ATTO 488 |
Research Area: |
Cancer | Cell Cycle | Cyclins |
Alternative Name: |
Cyclin Es Antibody, cyclin E variant ex5del Antibody, Cyclin E1 Antibody, Cyclin Et Antibody, CCNE1 Antibody, CCNE Antibody, G1/S-specific cyclin-E1 Antibody, cyclin E variant ex7del Antibody, CCNE1_HUMAN Antibody, G1/S specific cyclin E Antibody, Cy |
Category: |
Antibodies |
Product Type: |
Polyclonal Antibody |
Immunogen: |
Synthetic phospho-peptide from human Cyclin E1 around the phosphorylation site of threonine 395 (pThr395). |
Immunogen Species: |
Human |
Applications: |
WB, IHC, ELISA |
Host: |
Rabbit |
Isotype: |
IgG |
Species Reactivity: |
Human |
Antibody Dilution: |
WB (1:250-1:500); IHC (1:25-1:50); ELISA (1:5000); optimal dilutions for assays should be determined by the user. |
Purification: |
Peptide Affinity Purified |
Storage Buffer: |
PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide |
Concentration: |
N/A |
Specificity: |
Detects endogenous levels of Cyclin E1 only when phosphorylated at threonine 395 (pThr395). |
Storage: |
-20ºC |
Shipping: |
Blue Ice or 4ºC |
Certificate of Analysis: |
A 1:500 dilution of SPC-1367 was sufficient for detection of Cyclin E1 (pThr395) in 10 µg of human cervical cancer cell line (hela) lysate by ECL immunoblot analysis using goat anti-rabbit as the secondary antibody. |
Cellular Localization: |
Nucleus, Cytosol, Nucleoplasm, Cyclin E1-Cdk2 Complex |
Tissue Specificity: |
Highly expressed in testis and placenta. Low levels in bronchial epithelial cells. |
Accession Number: |
NP_001229.1 |
Gene ID: |
898 |
Swiss-Prot: |
P24864 |
Field of Use: |
For in vitro research use only. |