Cyclophilin B, Mouse Recombinant | P1197

PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1197 | Cyclophilin B Mouse Recombinant DataSheet

Biomolecule/Target: Ppib

Synonyms: AA408962, AA553318, AI844835, Cphn-2, Cphn2, CyP-20b

Alternates names: OB Protein, Obesity Protein, OBS, Obesity factor, HuB219, OB receptor, CD_antigen=CD295

Taglines: An immunophilin family protein catalyzing the isomerization of peptide bonds

NCBI Gene ID #: 3952

NCBI Gene Symbol: LEP

Gene Source: Mouse

Accession #: P48357

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Biological activity: N/A

Results: >1,000 nmol/min/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that cleaves 1 nmole of suc-AAFP-PNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight: N/A

Concentration: N/A

Appearance: Liquid

Physical form description: In Phosphate Buffered Saline (pH7.4) containing 10% glycerol

Reconstitution Instructions: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 g/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles

Amino acid sequence: Phe22-Asp839