CYP1B1 Antibody (Center) [APR32518G]

CYP1B1 Antibody (Center) [APR32518G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CYP1B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 143-176 amino acids from the Central region of human CYP1B1.

Cellular Localisation: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64429}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q64429}. Microsome membrane {ECO:0000250|UniProtKB:Q64429}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q64429}. Mitochondrion {ECO:0000250|UniProtKB:Q64429}. Note=Located primarily in endoplasmic reticulum. Upon treatment with 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD), CYP1B1 is also targeted to mitochondria {ECO:0000250|UniProtKB:Q64429}

Molecular Weight: 60846

Clone: Polyclonal

Gene Name: CYP1B1 {ECO:0000303|PubMed:8910454, ECO:0000312|HGNC:HGNC:2597}

Gene ID: 1545

Function: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:20972997, PubMed:11555828, PubMed:12865317, PubMed:10681376, PubMed:15258110) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:20972997, PubMed:11555828, PubMed:12865317, PubMed:10681376, PubMed:15258110) . Exhibits catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2- and 4-hydroxy E1 and E2. Displays a predominant hydroxylase activity toward E2 at the C-4 position (PubMed:11555828, PubMed:12865317) . Metabolizes testosterone and progesterone to B or D ring hydroxylated metabolites (PubMed:10426814) . May act as a major enzyme for all-trans retinoic acid biosynthesis in extrahepatic tissues. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376, PubMed:15258110) . Catalyzes the epoxidation of double bonds of certain PUFA. Converts arachidonic acid toward epoxyeicosatrienoic acid (EpETrE) regioisomers, 8, 9-, 11, 12-, and 14, 15- EpETrE, that function as lipid mediators in the vascular system (PubMed:20972997) . Additionally, displays dehydratase activity toward oxygenated eicosanoids hydroperoxyeicosatetraenoates (HpETEs) . This activity is independent of cytochrome P450 reductase, NADPH, and O2 (PubMed:21068195) . Also involved in the oxidative metabolism of xenobiotics, particularly converting polycyclic aromatic hydrocarbons and heterocyclic aryl amines procarcinogens to DNA-damaging products (PubMed:10426814) . Plays an important role in retinal vascular development. Under hyperoxic O2 conditions, promotes retinal angiogenesis and capillary morphogenesis, likely by metabolizing the oxygenated products generated during the oxidative stress. Also, contributes to oxidative homeostasis and ultrastructural organization and function of trabecular meshwork tissue through modulation of POSTN expression (By similarity) .

Summary: Tissue Location: Expressed in heart, brain, lung, skeletal muscle, kidney, spleen, thymus, prostate, testis, ovary, small intestine, colon, and peripheral blood leukocytes (PubMed:8175734) . Expressed in retinal endothelial cells and umbilical vein endothelial cells (at protein level) (PubMed:19005183) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Cytochrome P450 1B1, CYPIB1, CYP1B1