CYP24A1 Antibody (C-term) [APR32584G]

CYP24A1 Antibody (C-term) [APR32584G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CYP24A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 466-494 amino acids from the C-terminal region of human CYP24A1.

Cellular Localisation: Mitochondrion {ECO:0000250|UniProtKB:Q09128}.

Molecular Weight: 58875

Clone: Polyclonal

Gene Name: CYP24A1 (HGNC:2602)

Gene ID: 1591

Function: A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D (3) ) and the active hormone calcitriol (1-alpha, 25-dihydroxyvitamin D (3) ) (PubMed:24893882, PubMed:15574355, PubMed:8679605, PubMed:11012668, PubMed:16617161, PubMed:29461981) . With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:24893882, PubMed:15574355) . With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25 (OH) D3-26, 23-lactone as end product (PubMed:11012668, PubMed:8679605) . Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S- hydroxycholecalciferol and 20S, 23-dihydroxycholecalciferol (PubMed:25727742) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:8679605) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:500

Synonyms: 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial, 24-OHase, Vitamin D (3) 24-hydroxylase, Cytochrome P450 24A1, Cytochrome P450-CC24, CYP24A1, CYP24