CYP24A1 Antibody (N-term) [APR33160G]

CYP24A1 Antibody (N-term) [APR33160G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CYP24A1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 34-64 amino acids from the N-terminal region of human CYP24A1.

Cellular Localisation: Mitochondrion {ECO:0000250|UniProtKB:Q09128}.

Molecular Weight: 58875

Clone: Polyclonal

Gene Name: CYP24A1 (HGNC:2602)

Gene ID: 1591

Function: A cytochrome P450 monooxygenase with a key role in vitamin D catabolism and calcium homeostasis. Via C24- and C23-oxidation pathways, catalyzes the inactivation of both the vitamin D precursor calcidiol (25-hydroxyvitamin D (3) ) and the active hormone calcitriol (1-alpha, 25-dihydroxyvitamin D (3) ) (PubMed:24893882, PubMed:15574355, PubMed:8679605, PubMed:11012668, PubMed:16617161, PubMed:29461981) . With initial hydroxylation at C-24 (via C24-oxidation pathway), performs a sequential 6-step oxidation of calcitriol leading to the formation of the biliary metabolite calcitroic acid (PubMed:24893882, PubMed:15574355) . With initial hydroxylation at C-23 (via C23-oxidation pathway), catalyzes sequential oxidation of calcidiol leading to the formation of 25 (OH) D3-26, 23-lactone as end product (PubMed:11012668, PubMed:8679605) . Preferentially hydroxylates at C-25 other vitamin D active metabolites, such as CYP11A1-derived secosteroids 20S- hydroxycholecalciferol and 20S, 23-dihydroxycholecalciferol (PubMed:25727742) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via FDXR/adrenodoxin reductase and FDX1/adrenodoxin (PubMed:8679605) .

Summary: N/A

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial, 24-OHase, Vitamin D (3) 24-hydroxylase, Cytochrome P450 24A1, Cytochrome P450-CC24, CYP24A1, CYP24