CYP4A11 (4A22) Antibody (C-term) [APG02896G]

CYP4A11 (4A22) Antibody (C-term) [APG02896G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CYP4A11 (4A22) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 407-435 amino acids from the C-terminal region of human CYP4A11 (4A22) .

Cellular Localisation: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein

Molecular Weight: 59348

Clone: Polyclonal

Gene Name: CYP4A11 {ECO:0000303|PubMed:8274222, ECO:0000312|HGNC:HGNC:2642}

Gene ID: 1579

Function: A cytochrome P450 monooxygenase involved in the metabolism of fatty acids and their oxygenated derivatives (oxylipins) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:10553002, PubMed:10660572, PubMed:15611369) . Catalyzes predominantly the oxidation of the terminal carbon (omega-oxidation) of saturated and unsaturated fatty acids, the catalytic efficiency decreasing in the following order: dodecanoic > tetradecanoic > (9Z) -octadecenoic > (9Z, 12Z) -octadecadienoic > hexadecanoic acid (PubMed:10553002, PubMed:10660572) . Acts as a major omega-hydroxylase for dodecanoic (lauric) acid in liver (PubMed:7679927, PubMed:1739747, PubMed:8914854, PubMed:15611369) . Participates in omega-hydroxylation of (5Z, 8Z, 11Z, 14Z) -eicosatetraenoic acid (arachidonate) to 20- hydroxyeicosatetraenoic acid (20-HETE), a signaling molecule acting both as vasoconstrictive and natriuretic with overall effect on arterial blood pressure (PubMed:10620324, PubMed:10660572, PubMed:15611369) . Can also catalyze the oxidation of the penultimate carbon (omega-1 oxidation) of fatty acids with lower efficiency (PubMed:7679927) . May contribute to the degradation of saturated very long-chain fatty acids (VLCFAs) such as docosanoic acid, by catalyzing successive omega-oxidations to the corresponding dicarboxylic acid, thereby initiating chain shortening (PubMed:18182499) . Omega- hydroxylates (9R, 10S) -epoxy-octadecanoate stereoisomer (PubMed:15145985) . Plays a minor role in omega-oxidation of long-chain 3-hydroxy fatty acids (PubMed:18065749) . Has little activity toward prostaglandins A1 and E1 (PubMed:7679927) .

Summary: Tissue Location: Expressed in liver (PubMed:7679927) . Expressed in S2 and S3 segments of proximal tubules in cortex and outer medulla of kidney (PubMed:7679927, PubMed:10660572) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IF

Dilution: WB--1:1000 IF--1:10~50

Synonyms: Cytochrome P450 4A11, 20-hydroxyeicosatetraenoic acid synthase, 20-HETE synthase, CYP4AII, CYPIVA11, Cytochrome P-450HK-omega, Cytochrome P450HL-omega, Fatty acid omega-hydroxylase, Lauric acid omega-hydroxylase, CYP4A11, CYP4A2