CYP51A1 Antibody (Center) [APR10867G]

CYP51A1 Antibody (Center) [APR10867G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CYP51A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-279 amino acids from the Central region of human CYP51A1.

Cellular Localisation: Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q64654}; Single-pass membrane protein

Molecular Weight: 57278

Clone: Polyclonal

Gene Name: CYP51A1 (HGNC:2649)

Gene ID: 1595

Function: A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24, 25- dihydrolanosterol likely through sequential oxidative conversion of 14- alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14, 15 double bond in the sterol core and concomitant release of formic acid (PubMed:20149798, PubMed:8619637) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH- ferrihemoprotein reductase) (PubMed:20149798, PubMed:8619637) .

Summary: Tissue Location: Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: IHC-P, FC, WB

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: Lanosterol 14-alpha demethylase, LDM, CYPLI, Cytochrome P450 51A1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450LI, Sterol 14-alpha demethylase, CYP51A1, CYP51