CYR61, Human Recombinant | 7131

CYR61 is a member of the CCN family of secreted cysteine rich regulatory proteins. CYR61 induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells. Cell migration and adhesion are mediated through binding to specific cell surface integrins and to heparin sulfate proteoglycans. Increased expression of CYR61 is associated with several types of cancer, and correlates with the progression and estrogen independence of human breast cancers. Recombinant human CYR61 is a 39.4 kDa protein containing 357 amino acid residues. It is composed of four distinct structural domains (modules); the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the Thrombospondin type-I (TSP type-1) domain, and a C-terminal cysteine knot-like domain (CTCK).

7131 | CYR61 Human Recombinant DataSheet

Biomolecule/Target: CYR61

Synonyms: CCN1, G1G1 protein

Alternates names: Fibroblast Growth Factor-20, FGFK

Taglines: Induces angiogenesis by stimulating the proliferation, migration, and adhesion of endothelial cells

NCBI Gene ID #: 26281

NCBI Gene Symbol: FGF20

Gene Source: Human

Accession #: Q9NP95

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by the dose-dependent stimulation of the proliferation of murine 3T3 cells. The expected ED50 for this effect is 2.0-3.0 g/ml.

Biological activity: The ED as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is <10 ng/ml, corresponding to a specific activity of 1 x 10E5 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 23.2 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 10 mM Sodium Citrate, pH 3.0 and 50 mM NaCl.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A