Cytidine deaminase, human recombinant | 7363

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

7363 | Cytidine deaminase human recombinant DataSheet

Biomolecule/Target: Cytidine Deaminase

Synonyms: CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase

Alternates names: CDA, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase

Taglines: An enzyme that scavenges exogenous and endogenous cytidine and 2'-deoxycytidine for UMP synthesis

NCBI Gene ID #: UniGeneHs.419815

NCBI Gene Symbol: EGF

Gene Source: Human

Accession #: Q6QBS2

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 18.3 kDa (166 aa, 1-146 aa + His Tag).

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 1 mM DTT, 2 mM EDTA and 40% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ