Cytochrome c Antibody | 3352

Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.

3352 | Cytochrome c Antibody DataSheet

Antibody Target: Cytochrome c

Target Alternative Name: CYCS, CYC

Tag Line:

Category: Cytochrome

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: rat

Immunogen Sequence: Rat cytochrome c

Accession #: NP_036971

Gene ID: 6978725

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.2 mg/ml

Formulation: 100 µg (0.2 mg/ml) affinity purified rabbit anti-Cytochrome c polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Purification: Affinity purified

Application: Western blot

Positive Control:

Application And Usages: Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Rat kidney lysate (Cat.# 2702-100) can be used as a positive control. The cytochrome c antibody detects the 12 kDa cytochrome c from rat samples.

Country of Animal Origin: China

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified