D-amino acid oxidase, human recombinant | P1052

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

P1052 | D-amino acid oxidase human recombinant DataSheet

Biomolecule/Target: DAO

Synonyms: DAMOX, DAO, OXDA

Alternates names: apm-1, Adipolean , 30 kDa adipocyte complement-related protein, Adipocyte complement-related 30 kDa protein, Adipocyte, C1q and collagen domain-containing protein, Adipose most abundant gene transcript 1 protein, Gelatin-binding protein, ADIPOQ, ACDC, ACRP30, APM1, GBP28

Taglines: Enzyme that regulates the level of the neuromodulator D-serine in the brain.

NCBI Gene ID #: 9370

NCBI Gene Symbol: ADIPOQ

Gene Source: Human

Accession #: NM_004797

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

Biological activity: Determined by its ability to inhibit the proliferation of murine myeloid cell lines M1. The ED for this effect is < 1-3 µg/ml.

Results: > 3.5 units/mg

Binding Capacity: N/A

Unit Definition: One unit is the amount of enzyme that oxidatively deaminate 1.0 umole of D-alanine to pyruvate per minute at pH 8.5 at 37C, in the presence of catalase.

Molecular Weight: 16.6 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol

Reconstitution Instructions: Reconstitute Adiponectin/ gACRP30 protein in water to a concentration of 1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A