DAPK1 monoclonal Antibody | MB9177

DAPK1 monoclonal Antibody | MB9177 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Application: WB IHC

Application Range: WB:1:500 IHC:1:50-1:200

Background: Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Phosphorylates PIN1 resulting in inhibition of its catalytic activity, nuclear localization, and cellular function. Phosphorylates TPM1, enhancing stress fiber formation in endothelial cells. Phosphorylates STX1A and significantly decreases its binding to STXBP1. Phosphorylates PRKD1 and regulates JNK signaling by binding and activating PRKD1 under oxidative stress. Phosphorylates BECN1, reducing its interaction with BCL2 and BCL2L1 and promoting the induction of autophagy. Phosphorylates TSC2, disrupting the TSC1-TSC2 complex and stimulating mTORC1 activity in a growth factor-dependent pathway. Phosphorylates RPS6, MYL9 and DAPK3. Acts as a signaling amplifier of NMDA receptors at extrasynaptic sites for mediating brain damage in stroke. Cerebral ischemia recruits DAPK1 into the NMDA receptor complex and it phosphorylates GRINB at Ser-1303 inducing injurious Ca2+ influx through NMDA receptor channels, resulting in an irreversible neuronal death. Required together with DAPK3 for phosphorylation of RPL13A upon interferon-gamma activation which is causing RPL13A involvement in transcript-selective translation inhibition.

Storage & Stability: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Specificity: DAPK1 monoclonal Antibody detects endogenous levels of DAPK1 protein.

Molecular Weight: 160 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: DAP kinase 1, DAPK1, DAPK, DAK1, DAP K1, DAP kinase 1, DAPK 1, DAPK, DAPK1, DAPK1, Death Associated Protein Kinase 1, Death-associated protein kinase 1, DKFZp781I035 Antibody

Immunogen: Recombinant protein within Human DAP Kinase 1 aa 450-670.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: Protein affinity purified.

Pathway: