DARS polyclonal Antibody | BS7261

DARS polyclonal Antibody | BS7261 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human,Mouse,Rat

Application: WB,IHC

Application Range: WB 1:2000 - 1:5000 IHC 1:50 - 1:200

Background: Aminoacyl-tRNA synthetases consist of a family of enzymes that catalyze the specific aminoacylation of cognate tRNA in the initial step of ribosome-dependent protein biosynthesis. DaRS is part of a multisubunit complex of aminoacyl-tRNA synthetases and is involved in the transfer of Asp-tRNA to EF-1 α1 (elongation factor α 1) . The N-terminus of DaRS in vertebrates is a newly evolved structure that contains a putative amphiphilic helix and is dissimilar between different species. The N-terminal extension acts as a switch that, when in its stretched form, reduces the rate of dissociation of Asp-tRNA from DaRS, thereby providing enough time for EF-1 α1 to interact with Asp-tRNA. This suggests that the N-terminus of DaRS plays a critical role in its catalytic function. DaRS contains two phosphorylations sites, forms homodimers and localizes to the cytoplasm.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DARS polyclonal Antibody detects endogenous levels of DARS protein.

Molecular Weight: ~ 57 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Aspartate--tRNA ligase, cytoplasmic; Aspartyl-tRNA synthetase; AspRS; Cell proliferation-inducing gene 40 protein; DARS; PIG40

Immunogen: Recombinant full length Human DARS.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: