DC12 Antibody (C-term) [APR03512G]

DC12 Antibody (C-term) [APR03512G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DC12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human DC12.

Cellular Localisation: Chromosome. Note=Recruited to chromatin following DNA damage (PubMed:30554877) Localizes to replication forks (PubMed:30554877)

Molecular Weight: 40575

Clone: Polyclonal

Gene Name: HMCES {ECO:0000303|PubMed:30554877, ECO:0000312|HGNC:HGNC:24446}

Gene ID: 56941

Function: Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (PubMed:30554877, PubMed:31235915, PubMed:31235913) . Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA: forms a stable thiazolidine linkage between a ring-opened abasic site and the alpha-amino and sulfhydryl substituents of its N-terminal catalytic cysteine residue (PubMed:30554877, PubMed:31235913) . The HMCES DNA-protein cross-link is then degraded by the proteasome (PubMed:30554877) . Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (PubMed:30554877) . Has preference for ssDNA, but can also accommodate double-stranded DNA with 3' or 5' overhang (dsDNA), and dsDNA-ssDNA 3' junction (PubMed:31235915, PubMed:31806351) . Also involved in class switch recombination (CSR) in B-cells independently of the formation of a DNA- protein cross-link: acts by binding and protecting ssDNA overhangs to promote DNA double-strand break repair through the microhomology- mediated alternative-end-joining (Alt-EJ) pathway (By similarity) . Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (By similarity) .

Summary: N/A

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Embryonic stem cell-specific 5-hydroxymethylcytosine-binding protein, ES cell-specific 5hmC-binding protein, Putative peptidase SRAPD1, 34--, SRAP domain-containing protein 1, HMCES, C3orf37, DC12, SRAPD1