DCP2 Antibody (Center) [APR32666G]

DCP2 Antibody (Center) [APR32666G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This DCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the Central region of human DCP2.

Cellular Localisation: Cytoplasm, P-body. Nucleus Note=Predominantly cytoplasmic, in processing bodies (PB) (PubMed:15273322) . A minor amount is nuclear (PubMed:15273322)

Molecular Weight: 48423

Clone: Polyclonal

Gene Name: DCP2

Gene ID: 167227

Function: Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:12417715, PubMed:12218187, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550) . Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:12486012, PubMed:12923261, PubMed:21070968, PubMed:28002401, PubMed:31875550) . Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (PubMed:14527413) . Plays a role in replication-dependent histone mRNA degradation (PubMed:18172165) . Has higher activity towards mRNAs that lack a poly (A) tail (PubMed:21070968) . Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968) . The presence of a N (6) -methyladenosine methylation at the second transcribed position of mRNAs (N (6), 2'-O- dimethyladenosine cap; m6A (m) ) provides resistance to DCP2-mediated decapping (PubMed:28002401) . Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts (PubMed:26098573) .

Summary: Tissue Location: Expressed in brain and testis. Not detected in heart (at protein level) .

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P, FC

Dilution: WB--1:1000 IHC-P--1:100~500 FC--1:10~50

Synonyms: m7GpppN-mRNA hydrolase, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20, mRNA-decapping enzyme 2, hDpc, DCP2, NUDT20