DCTN1 Antibody | 18-125

DCTN1 Antibody | 18-125 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 945-1139 of human DCTN1 (NP_001128513.1) .

Research Area: Cell Cycle, Neuroscience, Signal Transduction

Tested Application: WB, IF, IP

Application: WB: 1:500 - 1:2000
IF: 1:20 - 1:100
IP: 1:50 - 1:100

Specificiy: N/A

Positive Control 1: U-251MG

Positive Control 2: HeLa

Positive Control 3: HepG2

Positive Control 4: 22RV1

Positive Control 5: Jurkat

Positive Control 6: Mouse testis

Molecular Weight: Observed: 150kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: DCTN1, dynactin 1 (p150, glued homolog, Drosophila) , P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, Glued (Drosophila) homolog)

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA) .