DDAH1, Human Recombinant | P1583

Dimethylarginine Dimethylaminohydrolase (DDAH) metabolizes asymmetric dimethyl arginine (ADMA) to L-citrulline and dimethylamine, and NG-monomethyl arginine (MMA) to L-citrulline and monomethylamine. Two isoforms, DDAH1 and DDAH2 have been found in mammalian cells. DDAH1 is widely expressed, especially in liver and kidney. DDAH2 predominates in vascular endothelium and expressed selectively in the kidney.

P1583 | DDAH1, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: DDAH-1, dimethylargininase-1

Alternates names: DDAH-1, dimethylargininase-1

Taglines: Regulates of nitric oxide generation

NCBI Gene ID #: 23576

NCBI Gene Symbol: DDAH1

Gene Source: Human

Accession #: O94760

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 5 mU/mg. Measured by its ability to hydrolyze asymmetric dimethylarginine to L-citrulline and dimethylamine by using BioVison’s DDAH Activity Fluorometric Assay Kit (Catalog # K2051)

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of DDAH is defined as the amount of enzyme that hydrolyzes 1 umol of asymmetric dimethyl arginine (ADMA) to L-citrulline per min at 37 °C under the assay conditions.

Molecular Weight: 31.4 KDa

Concentration:

Appearance: Liquid

Physical form description: In 50 mM Tris Buffer PH 8.0, 100 mM NaCl, 1 mM EDTA, 35% Glycerol

Reconstitution Instructions:

Amino acid sequence: aa 2-285

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans