DDB1 Antibody | 45-048

DDB1 Antibody | 45-048 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: Expected Species Reactivity based on sequence homology: Rat, Dog, Cow

Immunogen: The immunogen for this antibody is: C-DLIKVVEELTRIH

Research Area: Homeostasis

Tested Application: E, WB

Application: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 140kDa band observed in lysates of cell line HeLa, HepG2 and Jurkat (calculated MW of 126.9kDa according to Human NP_001914.3) . Recommended concentration: 1-2ug/ml. Approx 140kDa band observed in lysates of cell line NIH3T3 and approx 150kDa band observed in lysates of cell line NSO (calculated MW of 126.8kDa according to Mouse NP_056550.1) . Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Rat Kidney for this particular batch.

Specificiy: N/A

Positive Control 1: NSO Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD) , XPE

User Note: N/A

BACKGROUND: N/A